NMR identification of a partial helical conformation for bombesin in solution
- 1 February 1990
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 187 (3) , 645-650
- https://doi.org/10.1111/j.1432-1033.1990.tb15348.x
Abstract
The conformation of bombesin in trifluaroethanol/water mixtures has been studied using 1H-NMR spectroscopy. By a combination of two-dimensional 1H-NMR techniques and measurement of vicinal NH-.alpha.-CH spin-spin coupling constants, the secondary structure of the molecule has been determined. Bombesin adopts a helical structure in the region from Asn-6 to Met-14 with the remaining N-terminal portion existing as a more extended structure. The structure is very similar to that proposed from Fourier-transform infrared spectroscopic measurements for bombesin inserted into lipid bilayers [D. Erne and R. Schwayzer (1987) Biochemistry 26, 6316-6319]. The absence of a hydrogen bond between the sidechains of Trp-8 and His-12 is discussed in terms of the ionization state of His-12. Stabilisation of the helix results when His-12 is in the ionized state.This publication has 33 references indexed in Scilit:
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