Use of lacZ Gene Fusions to Determine the Dependence Pattern of the Sporulation Gene spoIID in spo Mutants of Bacillus subtilis

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Abstract
SUMMARY: The spoIID gene, which is involved in Bacillus subtilis sporulation, was fused to the β-galactosidase gene, lacZ, of Escherichia coli so that the expression of β-galactosidase would be under the control of the spoIID locus. When the fused product was inserted into the B. subtilis chromosome, production of β-galactosidase indicated that the spoIID gene was expressed 1.5 h after the start of sporulation. When the spoIID:: lacZ fusion was inserted into the chromosome of sporulation mutants, all strains carrying spoO lesions and those with mutations in spoIIA, spoIIE and spoIIG loci failed to make β-galactosidase. The proposed provisional order of expression of operons governing stage II is spoIIA [spoIIG, spoIIE] [spoIID, spoIIB, spoIIF].
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