AMMONIA FORMATION AND GLUTAMIG ACID IN BRAIN TISSUE

Abstract
During the incubation of brain homogenate of guinea pig there occurred NH3 formation, which was accompanied by a decrease in glutamic acid content as well as an increase in glutamine; the adenylic compounds determined by UV spectrophotometry at neighborhood of a260 m[mu]. was scarcely changed. Yield of NH3 was slightly reduced by the addition of 10-3 [image] inosinic and muscle adenylic acids, but considerably increased by inosinetriphosphate. In conclusion, glutamic acid is considered to be a predominant precursor of NH3 in brain tissue under the present condition.