Induction and Transplantation of Murine Skin Cancers Induced by Methoxsalen Plus Ultraviolet (320–400 nm) Radiation23

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Abstract
Properties of skin tumors resulting from treatment of mice with ip methoxsalen plus UVA (320–400 nm) radiation (PUVA) were compared to those of skin tumors induced by UVB (280–320 nm) radiation. Repeated treatment of C3H/HeN murine mammary tumor virus-negative (C3H−) mice with PUVA produced both fibrosarcomas and squamous carcinomas that were indistinguishable morphologically from UVB-induced tumors. Fragments of 12 primary PUVA-induced tumors were transplanted into normal and immunosuppressed syngeneic recipients, and all grew progressively. In contrast, 19 primary tumors induced by repeated exposure of C3H− mice to UVB grew only in the immunosuppressed mice and not in the normal recipients. The growth of graded doses of cells from 5 PUVA-induced tumors was compared in normal and UVB-irradiated recipients. No preferential growth in UVB-irradiated mice was observed, even though this is characteristic of UVB-induced tumors. Thus PUVA-induced tumors do not appear to have the same antigenic properties as UVB-induced tumors.