Adaptation of Equine Abortion Virus to Earle's L Cells in Serum-Free Medium with Plaque Formation.

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Abstract
L-M 929 strain cells were grown in serum-free YELP as monolayer cultures. Equine abortion virus, passage 109, in HeLa cells which had been frozen, thawed, and sonicated was used as the original inoculum. Subsequent 5-fold dilution passages of the virus revealed a characteristic cytopathic effect. Possible contamination by HeLa cells was ruled out by chromosomal studies and the toxicity of YELP to HeLa cells. Verification of the presence of the virus was accomplished by histologic and serologic methods, and by challenge of other host cells, i.e., hamster and HeLa. Preliminary studies of infectivity in this virus -cell system employing the tube method (TCID50) and agar overlay plaque method reveal titers of 10-7 to 10-8 to be readily reproducible.