Diphtheria toxin receptor-binding domain substitution with interleukin 6: genetic construction and interleukin 6 receptor-specific action of a diphtheria toxin-related interleukin 6 fusion protein

1 January 1991

journal article
Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection

Vol. 4 (8) , 989-994
https://doi.org/10.1093/protein/4.8.989

Abstract

We have genetically replaced that portion of the diphtheria toxin structural gene which encodes the native receptor-binding domain with a synthetic gene encoding the cytokine interleukin 6 (IL-6/IFN-β2/BSF-2). The resulting gene fusion encodes the chimeric toxin DAB₃₈₉-IL-6. Following expression and purification, we demonstrate that DAB₃₈₉-IL-6 is selectively cytotoxic for eukaryotic cells bearing the interleukin 6 receptor. In addition, the cytotoxic action of DAB₃₈₉-IL-6 is shown to require binding to the IL-6 receptor, internalization by receptor-mediated endocytosis and passage through an acidic compartment. Following the delivery of the catalytically active fragment A to the cytosol of target cells, cellular protein synthesis is inhibited by the ADP-ribosylation of elongation factor 2. While eukaryotic cells which are devoid of the IL-6 receptor are uniformly resistant to the action of this fusion toxin, the data presented suggest that a minimal number of IL-6 receptors may be necessary to mediate the internalization of sufficient levels of DAB₃₈₉-IL-6 to result in the intoxication of target cells.

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