In vitro evolution of new ribozymes with polynucleotide kinase activity
- 1 September 1994
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 371 (6492) , 31-36
- https://doi.org/10.1038/371031a0
Abstract
We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the γ-thiophosphate of ATP-γS to the 5′-hydroxyl or to internal 2′-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.Keywords
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