Streptococcal Desoxyribonuclease for the Removal of Feulgen-Stainable Material
- 1 January 1955
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 30 (1) , 9-11
- https://doi.org/10.3109/10520295509113729
Abstract
Removal of Feulgen-stainable material from the cell nucleus was accomplished by treatment of sections with streptococcal desoxyribonuclease. The procedure recommended is (1) Deparaffinize with xylene, followed by descending grades of alcohol. (2) Wash in tap water. (3) Treat slides for 1 hour at 37°C. with streptococcal desoxyribonuclease (1000 units/ml.) in 0.025M veronal buffer of pH 7.5 containing 0.003M MgSO4. Treat control slides for an equal length of time at the same temperature. Renew the enzyme approximately every 15 minutes. (4) Wash slides briefly in tap or distilled water. (5) Dehydrate, then coat the sections by dipping in a 1% solution of celloidin in alcohol-ether. (6) Subject the preparations to the Feulgen reaction. Control slides showed characteristic nuclear staining; enzyme treated slides did not stain.Keywords
This publication has 1 reference indexed in Scilit:
- The Specificity of the Feulgen Reaction for Thymonucleic AcidStain Technology, 1946