Simultaneous localization of type II collagen and core protein of chondroitin sulfate proteoglycan in individual chondrocytes.

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Abstract
In order to investigate the coordinated synthesis of matrix components by individual chick embryo chondrocytes, specific antibodies to type I collagen, type II collagen, and chondroitin sulfate proteoglycan core protein were used in simultaneous double immunofluorescence reactions. Extensive accumulation of core protein surrounding chondrocytes and the intracellular accumulation of type II collagen were observed. Extracellular core protein immunofluorescence obscured the intracellular reaction product, but the extracellular immunoreactive material could be removed by digestion with purified testicular hyaluronidase prior to fixation. Subsequent to digestion, core protein and type II collagen were observed in the same chondrocytes within discrete, sometimes identical, cytoplasmic regions, demonstrating the simultaneous localization of these 2 products characteristic of differentiating cartilage.

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