Abstract
The isolation of green sulfur bacteria from natural inocula, including one new species, Chlorobium thiosulfatophilum, is described. A synthetic medium which permits rapid growth and yields dense, healthy cultures has been developed. The new isolates are distinguished from the type species, Chlorobium limicola, by their ability to oxidize elementary S. C. thiosulfatophilum is physiologically characterized by its ability to utilize thiosulfate and tetrathionate as electron donors in photosynthesis. Evidence has been presented to show that both strains can use molecular H. The existing descriptions of C. limicola may be inadequate because they have been based on observations in "deficient" media.
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