Immunoelectrophoretic Analysis of Cytoplasmic Proteins of Neurospora crassa

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Abstract
Immunoelectrophoretic analysis (IEA) of cytoplasmic proteins of Neurospora crassa revealed more than 30 antigenic components in detectable concentrations in crude extracts. The IEA patterns varied somewhat with culture conditions and age, and with strain. A one-gene polymorphism was recognized but was not associated with any known biochemical or morphological mutant character. Comparison of the IEA patterns given by proteins of several species of fungi with antisera against N. crassa revealed the prominent phyletic affinities among the protein antigens within the class Ascomycetes. The results are discussed with emphasis on the methods and the interpretation of immunoelectro-phoretic variability.