Localization of Immunoglobulin and Viral Antigen in Rats Infected With Moloney Virus2

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Abstract
Equal numbers of normal rats and rats inoculated as newborns with Moloney virus were tested for their ability to produce antibovine serum albumin (BSA) antibody. Intracellular presence of BSA antibody and viral-associated antigen was followed by use of a double tag in the fluorescent antibody technique—a rhodamine tag to detect antibody and a fluorescein tag to detect virus. Under the conditions of the study, popliteal and lumbar lymph nodes were the main antibody-producing organs in both the infected and control rats. Four types of staining were seen on sections from infected rats: pure green cells indicating virus or viral-associated antigen production only, pure orange cells indicating antibody production only, and mixed green and orange stain, either as separate aggregates or homogeneous yellow, indicating both antibody and virus or viral-associated antigen production within the same cell. Discrete clones of orange or green cells were not seen; rather these cells intermingled. BSA titers and serum γ-globulin concentrations were lower in infected rats as compared to control rats. The amount of staining for viral antigen in the various organs of an individual infected rat was about the same for all organs examined except for the thymus where usually >75% of the cells were fluorescing green.

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