A Method for the Transfer of Tumorigenicity Between Strains of Agrobacterium tumefaciens in Carrot Root Disks

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Abstract
Surface-sterilized carrot root disks were inoculated concurrently with 0.1 ml each of a virulent (donor) and an avirulent (recipient) strain of A. tumefaciens cultures (1.2 .times. 109 colony-forming-units/ml). After 4-7 wk of contact between donor and recipient bacteria, 2 or 3 of 20 recipient colonies reisolated from tumor tissues became virulent. Transfer of tumorigenicity was not observed with recipient colonies reisolated from nontumor portions of tissue.

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