Plant regeneration from stem callus of cassava

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Abstract
Callus formation and organogenesis have been induced on the internode culture of cassava (Manihot esculenta Crantz). Callusing was rapidly induced on the medium devised by Kartha, Gamborg, and Constabel for the shoot apical meristem culture of cassava. During culture, green protuberances appear on the callus followed by the differentiation of a leaf-like structure. The leaf-like structure degenerated after a month of differentiation. On the same medium, but lacking gibberellic acid (GA3), organogenesis is less frequent and precocious but the leaves which appear are typical of cassava; leaf differentiation is followed by shoot development.

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