Acidemia and catecholamine output of the isolated canine adrenal gland

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Abstract
The isolated adrenal gland of the dog was perfused retrograde at constant flow at 37 C with diluted homologous blood at normal and acid pH. This medium preserved the integrity of the fine structure of the adrenal gland. The perfusion medium was made acid by equilibration with hypercapnic mixtures or addition of lactic acid. Norepinephrine and epinephrine concentrations were measured in the diluted blood after perfusion and adrenal catecholamine output (ACO) was calculated. At normal pH, ACO averaged 70 mg/gland/min. ACO was increased by 100% following perfusion with hypercapnic mixtures at a pH of 6.96-7.10 (PCO2 70-118 mm Hg) and 660% with a mixture at a pH of 6.79-6.92 (PCO2 125-210 mm Hg). This increase was primarily due to a rise in epinephrine concentration. Similar results were obtained following perfusion with media made acid by the addition of lactic acid. These results indicate that increases in [H+] directly stimulate adrenal medullary secretion. Acetylcholine, angiotensin, and tyramine injected into the perfusion fluid also produced marked increases in ACO.

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