A Rapid High Performance Liquid Ch80Matography System for the Separation of Gonadal Steroids

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Abstract
A radial compression reverse phase (C-18) column system in conjunction with a high performance liquid chromatograph has been developed which separates eighteen different gonadal steroids in a single run of forty minutes. In addition, three other gonadal steroids co-elute with two of the original eighteen but they could be separated by rechroraatography on an alkyl (C-8) HPLC column. This combination of two columns allows the complete separation of twenty-one gonadal steroids or metabolites of the progesterone, pregnenolone, 5-α reductase, and aromatose pathways in less than one hour compared to a few weeks required when thin layer and paper chromatorgraphy are used to separate these steroid as in the present method employed.