Altered dihydrofolate reductase in fol regulatory mutants of Escherichia coli K12
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 151 (2) , 215-219
- https://doi.org/10.1007/bf00338697
Abstract
Three spontaneous fol regulatory mutants contain dihydrofolate reductase molecules which differ in physical properties from enzymes of their parent strains. The enzymes were purified over 100-fold by affinity chromatography and were shown to differ in vitro in thermolability and in affinity for trimethoprim, a competitive inhibitor of the enzyme. These results indicate that some fol regulatory mutations occur in the structural gene for dihydrofolate reductase.This publication has 15 references indexed in Scilit:
- A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4Journal of Molecular Biology, 1976
- Regulatory mutants of dihydrofolate reductase in Escherichia coli K12Molecular Genetics and Genomics, 1976
- Mutants which allows accumulation of tRNATyr precursor molecules.1975
- Evidence for post-transcriptional control of the morphogenetic genes of bacteriophage lambdaJournal of Molecular Biology, 1974
- Cleavage by RNase III Converts T3 and T7 Early Precursor RNA into Translatable MessageProceedings of the National Academy of Sciences, 1974
- Autogenous Regulation of Gene ExpressionScience, 1974
- [22] Methotrexate-agarose in the purification of dihydrofolate reductasePublished by Elsevier ,1974
- Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coliBiochemistry, 1972
- Secondary structure of bacteriophage f2 ribonucleic acid and the initiation of in vitro protein biosynthesisJournal of Molecular Biology, 1970
- Increased dihydrofolate reductase synthess in Diplococcus pneumoniae following translatable alteration of the structural gene. I. Genotype derivation and recombinational analyses.1969