Isolation of autophagocytosis mutants of Saccharomyces cerevisiae
- 1 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 349 (2) , 275-280
- https://doi.org/10.1016/0014-5793(94)00672-5
Abstract
Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.Keywords
This publication has 26 references indexed in Scilit:
- Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiaePublished by Wiley ,2001
- Site of catabolite inactivationNature, 1994
- Vacuolar/lysosomal proteolysis: proteases, substrates mechanismsCurrent Opinion in Cell Biology, 1993
- Biogenesis of the yeast vacuole (lysosome)European Journal of Biochemistry, 1992
- Ubiquitin, a central component of selective cytoplasmic proteolysis, is linked to proteins residing at the locus of non‐selective proteolysis, the vacuoleFEBS Letters, 1992
- Autophagy and other vacuolar protein degradation mechanismsCellular and Molecular Life Sciences, 1992
- Biogenesis of the yeast vacuole (lysosome)European Journal of Biochemistry, 1992
- Regulated import and degradation of a cytosolic protein in the yeast vacuoleNature, 1991
- Plantacin B, a bacteriocin produced byLactobacillus plantarumNCDO 1193FEMS Microbiology Letters, 1988
- On the Structure of Fatty Acid Synthetase of YeastEuropean Journal of Biochemistry, 1980