RELATIONSHIP OF HEMOGLOBIN-SYNTHESIS TO ERYTHROID COLONY AND BURST FORMATION

Google Scholar
  • 1 January 1979
    • journal article
    • research article
    • Vol. 53  (5) , 935-945
Abstract
The cyclohexanone method for the extraction of hematin was used to measure Hb synthesis induced by erythropietin (epo) in mouse bone marrow cells cultured in medium containing methyl cellulose. The time course of Hb synthesis by mouse narrow cells showed 2 effects due to epo: an increase in Hb synthesis at day 2, which correspond to the formation of small erythroid colonies resulting from the CFU-E (colony-forming unit, erythroid), and a very large increase in Hb synthesis, which was maximal at days 7-8 and corresponded to the formation of large erythroid colonies (bursts) resulting from the BFU-E (burst-forming unit, erythroid). The epo dose-response curves for the CFU-E colony counts and day-2 Hb synthesis were similar and the cell-number-response curves for the 2 parameters were parallel. The epo dose-response curve for BFU-E colony counts reached a plateau at an epo concentration between 3-5 units/ml, whereas the dose-response curve for 6-8 day Hb synthesis did not reach a plateau even at an epo dose of 10 units/ml.