Identifizierung des acyclischen Zwischenproduktes der Nicotinsäure-Biosynthese, IV. Umwandlung des Zwischenproduktes in ε-Hydroxy-β-carboxy-norleucin und β-Carboxy-lysin

Publisher Website
Google Scholar
Abstract
The structure of the acyclic biosynthetic precursor of nictotinic acid was elucidated. The intermediate was prepared by the enzymic oxidation of 3-hydroxyanthranilic acid, purified, then reacted with dinitro-phenylhydrazine. Spectrophotometric tests showed that the intermediate was unchanged by the methods employed. The intermediate was hydrogenated to [epsilon]-hydroxy-[beta]-carboxynorleucine, and its dinitro-phenylhydrazone was hydrogenated to[beta] -carboxylysine. These 2, hitherto unknown amino acids were synthesized and their diastereomers were separated. The amino acids isolated from enzymic reaction mixtures were identical with the synthetic amino acids. It was proved that the acyclic precursor has the structure of an [alpha]-amino-[beta]-carboxy-muconic acid-[epsilon]-semialdehyde.

This publication has 13 references indexed in Scilit: