Glucose Metabolism in Sediments of a Eutrophic Lake: Tracer Analysis of Uptake and Product Formation
- 1 December 1982
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 44 (6) , 1308-1317
- https://doi.org/10.1128/aem.44.6.1308-1317.1982
Abstract
The uptake of glucose and the formation of end products from glucose catabolism have been measured for sediments of eutrophic Wintergreen Lake with a combination of tritiated and 14 C-labeled tracers. Time course analyses of the loss of [ 3 H]glucose from sediments were used to establish rate constants for glucose uptake at natural substrate concentrations. Turnover times from these analyses were about 1 min for littoral and profundal sediments. No seasonal or site differences were noted in turnover times. Time course analyses of [ U - 14 C]glucose uptake and 14 C-labeled end product formation indicated that glucose mass flow could not be calculated from end product formation since the specific activity of added [ 14 C]glucose was significantly diluted by pools of intracellular glucose and glucose metabolites. Mass flow could only be accurately estimated by use of rates of uptake from tracer studies. Intermediate fermentation end products included acetate (71%), propionate (15%), lactate (9%), and only minor amounts of butyrates or valerates. Addition of H 2 to sediments resulted in greater production of lactate (28%) and decreased formation of acetate (50%), but did not affect glucose turnover. Depth profiles of glucose uptake indicated that rates of uptake decreased with depth over the 0- to 18-cm interval and that glucose uptake accounted for 30 to 40% of methanogenesis in profundal sediments.This publication has 8 references indexed in Scilit:
- Intermediary Metabolism of Organic Matter in the Sediments of a Eutrophic LakeApplied and Environmental Microbiology, 1982
- Reduction of Sulfur Compounds in the Sediments of a Eutrophic Lake BasinApplied and Environmental Microbiology, 1981
- Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence of Methanobacterium thermoautotrophicumApplied and Environmental Microbiology, 1977
- Inhibitory effects of H2 on growth of Clostridium cellobioparumApplied and Environmental Microbiology, 1976
- Glucose flux at the sediment–water interface of Toronto Harbour, Lake Ontario, with reference to pollution stressCanadian Journal of Microbiology, 1973
- Method for measuring mineralization in lake sediments.1971