Effect of Incubation Medium Electrolyte Concentration on Lens Volume

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Abstract
In rat lenses that are incubated in anisotonic media, the initial tendency to change volume is countered by a change in the intralenticular concentration of solute so that the amount of volume change is minimized. The effect of electrolytes in the medium on solute concentration and content was determined by comparing the effects of two series of media. In one osmolarity was varied by changing the concentration of sodium chloride and in the other the osmolarity was varied by changing the concentration of sucrose. The results indicate that volume regulation in the rat lens is a dual process with the content of lens electrolytes varying with the electrolyte concentration in the medium and with the content of ‘other solute’ varying with osmolarity. Potassium fluxes were estimated from 86Rb influx and efflux measurements. When the osmolarity is varied by changing the concentration of sodium chloride the fluxes increase with increasing osmolarity.

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