Detection of Baculovirus Protein in Cell Culture and Insect Larvae by Enzyme-linked Immunosorbent Assay (ELISA)

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Abstract
Cell cultures of the alfalfa looper, Trichoplusia ni (Tn-368), codling moth, Laspeyresia pomonella (Cp-169) and viper spleen cells (viper-VSW) were inoculated with Autographa californica non-occluded nuclear polyhedrosis virus (AcMNPV-NOV). In Tn-368 and Cp-169 cells an increase in the concentration of virus protein was detected by enzyme-linked immunosorbent assay 9-20 h after virus inoculation. An average of 2 ng AcMNPV protein was detected in homogenates of T. ni larvae 12-14 h post-infection (p.i.), Heliothis zea nuclear polyhedrosis virus (HzSNPV) proteins were first detected in H. zea larvae 16 h p.i. At this time .apprx. 60 ng HzSNPV protein was detected in homogenates of the virus-infected larvae. Virus protein was not detected in viper-VSW and Lymantria dispar larvae inoculated with AcMNPV or in Estigmene acrea larvae inoculated with HzSNPV.