The potential of dielectrophoresis for single-cell experiments
- 1 November 2003
- journal article
- research article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Engineering in Medicine and Biology Magazine
- Vol. 22 (6) , 51-61
- https://doi.org/10.1109/memb.2003.1266047
Abstract
In this article, a microfluidic system was developed for sorting and high-content analysis of single cells using dielectrophoresis. It makes high-resolution imaging of suspended cells feasible immediately before depositing them into a microtiter plate. The microfluidic devices contain three-dimensional arrangements of radio-frequency driven electrodes and are used to gently manipulate suspended cells in flow. Viability of sorted and deposited cells is comparable to a control group. The influence of RF electric fields is studied using (a) calcium flux measurements and membrane potential dependent fluorescence dye on suspended human cells and (b) cultivation of yeast trapped for several days in dielectric field cages driven at field strengths up to 100 kV/m and 7 MHz.Keywords
This publication has 26 references indexed in Scilit:
- Microdevices for manipulation and accumulation of micro‐ and nanoparticles by dielectrophoresisElectrophoresis, 2003
- Trapping in AC octode field cagesJournal of Electrostatics, 2000
- Paired microelectrode system: dielectrophoretic particle sorting and force calibrationJournal of Electrostatics, 1999
- Electro-rotation in octopole micro cagesJournal of Physics D: Applied Physics, 1999
- Separation of Submicron Bioparticles by DielectrophoresisBiophysical Journal, 1999
- A 3-D microelectrode system for handling and caging single cells and particlesBiosensors and Bioelectronics, 1999
- Ac electrokinetics: a review of forces in microelectrode structuresJournal of Physics D: Applied Physics, 1998
- Dielectrophoretic Sorting of Particles and Cells in a MicrosystemAnalytical Chemistry, 1998
- Equilibria and dynamics of DEP-levitated particles: multipolar theoryJournal of Electrostatics, 1994
- Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regimeBiophysical Journal, 1992