Near-neighbor relations of the subunits of cytochrome c oxidase

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Abstract
Cytochrome c oxidase [beef heart] in detergent dispersion was cross-linked with 2 reversible cross-linking agents, dithiobissuccinimidylpropionate (DSP) and dimethyl-3,3''-dithiobispropionimidate (DTBP), and the cross-linked products formed were analyzed by 2-dimensional gel electrophoresis. Under mild reaction conditions, several subunit pairs were seen. With higher levels of DSP, larger aggregates were seen until a cross-linked product with an apparent MW of 140,000 was the predominant band on gels. This is the smallest molecular weight aggregate to contain all 7 subunits of the enzyme and most likely represents the unit or 2 heme and 2 Cu containing complex of cytochrome c oxidase.