Survival of Mice in Absence of Inert Gas.

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Abstract
In order to test biological function in the absence of an inert gas such as N2, mice were maintained in a chamber for as long as 55 days in an atmosphere of O2 at a reduced pressure of 197 mm Hg. CO2 or N2 pressures did not exceed 5 mm Hg during this exposure. Usually animals did not encounter any difficulties. Several litters were born and in one experiment young were conceived, born and raised in the virtual absence of inert gas. However, in several cases animals died within 48 hours of their initial exposure. Once past this period only mechanical failure of the apparatus terminated these experiments. It is suggested that pulmonary atelectasis was responsible for these early deaths.

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