Factors affecting the catalytic action of copper in the oxidation of cysteine

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Abstract
Pyrophosphate increases the O uptake of a system of cysteine and heart muscle preparation in orthophosphate buffer pu 7.6. If dilute Cu salts replace the muscle preparation, the acceleration is obtained. The optimum activity of Cu in phosphate buffers containing pyrophosphate occurs at p H 8.0 at 20[degree] C. Dilute H2S inhibits the activity of Cu in phosphate buffers containing pyrophosphate, at P H 7.0, as much as 60%. The inhibition decreases as the PH increases and at P H 8.0 there is very little inhibition. Relatively large amounts of Fe inhibit the activity of Cu in the presence of pyrophosphate. This effect of Fe is eliminated if the temp. is increased to 37[degree] and the more concentrated solutions of pyrophosphate used.