Comparison of isozyme variation among natural stands and plantations: jack pine and black spruce

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Abstract
Isozymes from needles of jack pine (Pinusbanksiana) and black spruce (Piceamariana) were analysed to test differences among young stands, mature stands, plantations from seed-zone seed collections, a plus-tree clonal seed orchard, and a progeny plantation from the seed orchard. Analyses based on approximately 100 trees per population resolved seven polymorphic loci for jack pine and five for black spruce. For intraspecies comparisons, allelic heterogeneity tests indicated no differences between young stands and plantations from seed-zone seed collections. The black spruce clonal seed orchard and mature natural stands were genetically homogeneous. Allelic heterogeneity test and discriminant analysis indicated that the progeny plantation from the clonal seed orchard differed from other black spruce samples. It is proposed that nonrandom mating conditions in the clonal seed orchard altered allele frequencies of the resultant progeny plantation.

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