LEUKOCYTE MIGRATION IN DIFFERENT SYSTEMS - EFFECT OF COLCHICIN - KINETICS OF MIGRATION UNDER AGAROSE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 88  (2) , 89-96
Abstract
In a double-filter modification of Boyden''s method, formylmethionylleucylphenylalanine (FMLP) needed the presence of fresh plasma to induce [human] leukocyte locomotor kinetics which compared with a model for directional migration. FMLP, or zymosan-activated plasma, did not stimulate migration in capillary tubes. Kinetics of leukocyte migration under agarose towards zymosan-activated plasma were more compatible to a model for increased random motility than for directional migration. Treatment of leukocytes with colchicine reduced their migration under gradient and non-gradient conditions in millipore filters, capillary tubes and under-agarose experiments.