Properties of antisera against lymphocytes of nude mice

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Abstract
The activity of rabbit antisera against nu/nu BALB/c lymphocytes was estimated in vivo and in vitro.It was found that anti‐lymphocyte serum (ALS) against nu/nu lymph node cells suppressed the alloantigen reaction and the spontaneous rosette‐forming cell (sRFC) or plaque‐forming cell (PFC) formation for T‐dependent (sheep red blood cells) and T‐independent (lipopolysaccharide) antigens. ALS against nu/nu spleen cells affected only the sRFC and PFC for T‐independent antigen. The former serum exhibited a high cytotoxicity for the suspensions enriched or depleted in B cells, while the latter was more cytotoxic for the suspension enriched in B cells. This may indicate that ALS anti‐nu/nu spleen cells is specific for B lymphocytes, and ALS anti nu/nu lymph node cells is directed not only to B cells but also to a subpopulation of T lymphocytes. It may suggest the existence of a subpopulation of T lymphocytes in nu/nu lymph node cells.