A Simple Technique for Assaying Certain Microbial Phytotoxins and its Application to the Study of Toxins Produced by Spiroplasma citri

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Abstract
A method for detecting and assaying phytotoxins was developed which depends on damage caused by toxins to cells in leaves of broad bean [vicia faba] plants and the consequent production of black polyphenol oxidation products. The assay, which has the advantage of being relatively unaffected by components of complex microbial growth media, was used to study the properties of a toxin produced by the plant-pathogenic mycoplasma S. citri. The toxin is an unstable, acidic, polar compound of low MW.

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