Comparative Study of Different Extractive Procedures to Quantify Morphine in Urine by HPLC-UV

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Abstract
A comparative study to extract and purify total morphine in urine is described using several procedure treatments of biological samples. Sep-Pak C18 cartridges, Extrelut columns and liquid-liquid conventional extraction with sequencial purification through Extrelut column were assayed in order to establish the best procedure to quantitatively extract morphine from urine free of endogenous interferents. Analysis of the drug was made by HPLC-UV with a C18 column and methanol: acetate buffer (pH6.9) (70+30) as eluent. Recovery of morphine from spiked urine was 88.0% and 79.8% for concentrations of 1μg/ml and 5μg/ml, respectively. The detection limit of the method was 0.2μg/ml and coeficient of variation was 1.8% (n+4).