The uptake and metabolism of [32P]pyrophosphate by mouse calvaria in vitro
- 15 May 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 140 (2) , 175-183
- https://doi.org/10.1042/bj1400175
Abstract
In order to study the uptake and metabolism of PP(i) by bone, (32)PP(i) was added to the medium surrounding explanted mouse calvaria maintained in organ culture. Most of the PP(i) was hydrolysed during incubation, but there was a measurable entry of intact PP(i) into bone. When (32)P(i) was added to the medium, synthesis of PP(i) and organic phosphates from P(i) was observed in bone. There was no detectable passage of PP(i) from bone into the medium. These results are discussed in terms of two models of pyrophosphate hydrolysis and exchange. Some quantitative estimates about the fate of PP(i) in bone were made.Keywords
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