The enzymic estimation of glutamate and glutamine
- 1 April 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 99 (1) , 246-252
- https://doi.org/10.1042/bj0990246
Abstract
A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0-0.3 [mu]mole of glutamic acid. The response is linear, but not stoichiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.This publication has 13 references indexed in Scilit:
- Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotideBiochemical Journal, 1966
- THE NATURE OF THE RADIATION-INDUCED LESION OF THE ELECTRON TRANSPORT CHAIN OF THYMUS MITOCHONDRIACanadian Journal of Biochemistry, 1964
- Glutamate-dehydrogenase inactivation by reduced nicotinamide-adenine dinucleotide phosphateBiochemical Journal, 1962
- BESTIMMUNG VON L-LACTAT, L-MALAT, L-GLUTAMAT UND ATHYLALKOHOL IM ENZYMATISCH-OPTISCHEN TEST MIT HILFE DES DPN-ANALOGEN 3-ACETYL-PYRIDIN-DPN1962
- Sites of Electron Transfer to Tetrazolium Salts in the Succinoxidase SystemJournal of Biological Chemistry, 1960
- The substrate specificity of glutamic acid dehydrogenaseArchives of Biochemistry and Biophysics, 1960
- A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenasesArchives of Biochemistry and Biophysics, 1959
- Studies on the succinate–neotetrazolium reductase system. Activation by vitamin K3Biochemical Journal, 1959
- KINETIC AND EQUILIBRIUM STUDIES ON CRYSTALLINE l-GLUTAMIC ACID DEHYDROGENASEJournal of Biological Chemistry, 1953
- Phenazine compounds as carriers in the hexosemonophosphate systemBiochemical Journal, 1938