Imino‐proton resonances of yeast tRNAPhe studied by two‐dimensional nuclear Overhauser enhancement spectroscopy
Open Access
- 1 June 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 149 (3) , 649-655
- https://doi.org/10.1111/j.1432-1033.1985.tb08973.x
Abstract
Application of two‐dimensional nuclear Overhauser enhancement (NOE) spectroscopy to yeast tRNAPhe in H2O solution demonstrates that all imino‐proton resonances, related to the secondary structure, and nearly all imino proton resonances, originating from the tertiary structure, can be assigned efficiently by this method. The results corroborate the assignments of the imino‐proton resonances of this tRNA as established previously by one‐dimensional NOE experiments (only the assignment of base pairs G1· C72 and C2· G71 should be reversed). The advantages of two‐dimensional NOE spectroscopy over one‐dimensional NOE spectroscopy for the assignments of imino‐proton resonances and the structure elucidation of tRNA are illustrated and discussed. Furthermore, the use of non‐exchangeable proton resonances as probes of the molecular structure is explored.This publication has 25 references indexed in Scilit:
- Effective water resonance suppression in 1D‐ and 2D‐FT–1H‐nmr spectroscopy of biopolymers in aqueous solutionBiopolymers, 1983
- Assignment of imino proton spectra of yeast phenylalanine transfer ribonucleic acidBiochemistry, 1983
- Nuclear Overhauser assignment of the imino protons of the acceptor helix and the ribothymidine helix in the nuclear magnetic resonance spectrum of Escherichia coli isoleucine transfer ribonucleic acid: evidence for costacked helices in solutionBiochemistry, 1982
- Sequential resonance assignments in protein 1H nuclear magnetic resonance spectraJournal of Molecular Biology, 1982
- Sequential resonance assignments in protein 1H nuclear magnetic resonance spectraJournal of Molecular Biology, 1982
- Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonanceJournal of Molecular Biology, 1982
- Data shift accumulation and alternate delay accumulation techniques for overcoming the dynamic range problemJournal of Magnetic Resonance (1969), 1980
- Further refinement of the structure of yeast tRNAPheJournal of Molecular Biology, 1978
- Optimised parameters for A-DNA and B-DNABiochemical and Biophysical Research Communications, 1972
- Large-scale preparation of yeast “soluble” ribonucleic acidBiochemical and Biophysical Research Communications, 1963