Nitrogen Content of Dental Pulp

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Abstract
Total N has been used as a standard of reference for other tissues and it was decided to determine its concentration in dental pulp by macro-Kjeldahl, micro-Kjeldahl and nesslerization methods. Because fresh pulp is not always available for analysis the effects of formalin preservation on N analysis were also considered. Tissues studied were normal bovine pulps from animals between 2 and 3 years old, human infant pulps secured at autopsy and pulps of normal teeth from patients between 14 and 48 years old. Results of 304 total N determinations are reported. Bovine pulps were removed from the teeth, dried or fixed in 10% formalin and then dried. N content of fresh and preserved tissues determined by macro-and micro-Kjeldahl methods was not significantly different. Analysis by nesslerization yielded slightly lower N values but the difference between fresh and preserved pulps was negligible. Formalin preserved human infant and adult pulps analyzed by the micro-Kjeldahl method showed almost identical N contents. The mean total N (Kjeldahl) of human dental pulp was observed to be 0.1050 mg/mg of dried tissue and that of bovine pulp 0.1258 mg.

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