Studies of fatty acid oxidation. 2. The effect of alkylthio fatty acids on acetylation reactions

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Abstract
Sodium ethylthioacetate inhibits the oxidation of [alpha-C14] pyruvate to C14O2 and acetoacetate in guinea pig-liver slices but has no effect on the oxidation of [carboxy-C14] pyruvate to C14O2. The production of acetoaceate from acetate and butyrate is inhibited by sodium ethylthioacetate but C14O2 production from the [carboxy-C14]-labeled substrates is relatively unaffected. The effect of alkylthio fatty acids on acetoacetate formation is similar to that of benzoate. The simple and alkylthio fatty acids inhibit sulfanilamide acetylation with pyruvate as acetyl donor. Acetate increases the rate of acetylation. Alkylthio fatty acids form coenzyme A derivatives and evidence was obtained which supports the suggestion that both alkylthio fatty acids and benzoic acid produce their inhibitory effects on acetoacetate synthesis only after formation of a coenzyme A derivative.