INTERFERON-INDUCED CELL-CYCLE CHANGES IN HUMAN HEMATOPOIETIC-CELL LINES AND FRESH LEUKEMIC-CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (6) , 2358-2362
Abstract
A panel of 26 human hematopoietic cell lines was tested for sensitivity to growth inhibition towards interferon-.alpha. (IFN-.alpha.) by estimating the effects on cell cycle phase distribution using flow cytometry analysis. The proportion of proliferating cells was assessed by studying the fractional increase of cells in mitosis during a 24-h vinblastine block. Of 26 cell lines tested, 17 were sensitive to IFN-.alpha., and the main cell cycle effect was accumulation in the G0-G1 phase. One Burkitt''s lymphoma line, Namalwa, showed a decreased rate of progress through S without any G0/G1 accumulation. Three cell lines were also tested with IFN-.beta. and with IFN-.alpha.2 produced by recombinant DNA technology. The latter IFN did not affect 1 of the cell lines; otherwise, the results were similar to those of IFN-.alpha.. Six of 16 clinical specimens from patients with hematopoietic neoplasias were IFN-sensitive, all displaying a G0-G1 block. IFN sensitivity is an individually linked property unrelated to cell origin.