Abstract
The author gives a resume of the literature and points out that certain investigators definitely associate water leptospira with the etiology of specific cases of infectious jaundice. He found leptospiras in the tap water of Boston, in water from melting ice in the refrigerator, and in pools and ponds. These spirochaetes were cultivated in 2% sterilized human feces mixed with the water sample. He isolated Leptospira biflexa by a method which he describes, using Berkefeld candle grade "N." In 40 filtration experiments, 37 pure cultures of spirochaetes were obtained. A survey of 40 municipal water supplies indicates that slow sand filtration and use of chlorine inhibits growth of leptospiras. These spirochaetes are not pathogenic for laboratory animals, neither do they protect them against a virulent strain of Leptospira icterohemorrhagiae.

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