Excitation‐contraction coupling in skeletal muscle fibers injected with the InsP3blocker, heparin
- 1 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 235 (1-2) , 57-62
- https://doi.org/10.1016/0014-5793(88)81233-x
Abstract
Heparin, an inhibitor of inositol trisphosphate (InsP3)-induced Ca2+ release in smooth muscle and non-muscle cells, was injected into intact frog skeletal muscle fibres. Ca2+ release from the sarcoplasmic reticulum was elicited by the normal action potential mechanism and monitored by both fura-2 fluorescence and an intrinsic birefringence signal. Both optical signals, and hence Ca2+ release, were unaffected by high concentrations of heparin. This result argues against a major physiological role of InsP3 as a chemical messenger of excitation-contraction coupling in skeletal muscleKeywords
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