Radioimmunoassay of bile acids in serum.
Open Access
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 22 (5) , 602-606
- https://doi.org/10.1093/clinchem/22.5.602
Abstract
We developed four radioimmunoassay procedures for the determination of glycine-conjugated bile acids in serum. Antibodies to two primary bile acids, cholylgycine and chenodeoxycholylglycine, and to two secondary bile acids, sulfolithocholylglycine and deoxycholylglycine, were raised in rabbits after the acids were covalently linked to albumin by use of the carbodiimide reaction. Assay sensitivity for each of these bile acids is in the picomole range with the standard curve extending from 10-80 pmol. The concentration of bile acids in serum increased in various states of liver disease and its measurement appears to be an extremely sensitive indicator of liver function.This publication has 2 references indexed in Scilit:
- The Serum Trihydroxy-Dihydroxy Bile Acid Ratio in Liver and Biliary Tract Disease1Journal of Clinical Investigation, 1958
- Bile Acid Content of Human Serum. I. Serum Bile Acids in Patients with Hepatic Disease1Journal of Clinical Investigation, 1957