STUDIES ON THE NATURE OF ANTIBODY PRODUCTION DURING THE IN VITRO CULTURE OF LYMPHOID TISSUES

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Abstract
SUMMARY: A simple and reproducible technique for the study of the production of antibodies by lymphoid tissues during in vitro culture has been described.Cellular and extra‐cellular fluid titres were estimated by Boyden's tanned cell technique, and a sharp rise in the fluid antibody titre (up to 300‐fold) was observed during the first 18 to 24 hours of culture, while the cellular titre remained at a low constant level. The results of this study suggest that antibody‐producing cells store little or no classical antibody.The presence of chloramphenicol in the culture medium inhibited antibody production, while production was not specifically inhibited by ribonuclease. In addition, antibody production was inhibited by the absence of Ca++ from the culture medium and this effect was reversed when Ca++ was restored in physiological concentration.Attempts to stimulate antigenically spleen and popliteal node tissues during in vitro cultures were not successful.
Funding Information
  • National Health and Medical Research Council, Canberra

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