Mass Isolation of Malaria Sporozoites from Mosquitoes by Density Gradient Centrifugation

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Abstract
Summary Aedes aegypti mosquitoes, infected with Plasmodium gallinaceum, were homogenized and the resulting brei was subjected to a series of filtration and centrifugation steps prior to being placed on a linear sucrose gradient. The mean density of the sporozoites isolated from the peak fraction was 1.157 g/cm3 and the number of sporozoites recovered per mosquito was estimated to be 45,000. The technique can readily be extended to the isolation of sporozoites of other plasmodial species and produces a greater yield than is possible by conventional dissection methods.

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