NUTRITION OF THE PARASITIC PHASE OF COCCIDIOIDES IMMITIS IN A CHEMICALLY DEFINED LIQUID MEDIUM

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Abstract
Optimal spherule formation occurred in atmospheres containing 20% oxygen. In defined medium with low total solids content, a requriement for added CO2 was not demonstrated. Stimulation of spherulation resulted from the addition of (a) sodium and calcium ions, (b) sodium bicarbonate, (c) the sulfhydryl compound glutathione, (d) the fatty acids, oleic and linoleic, and (e) the action of such metabolic inhibitors as sodium fluoride, copper sulfate, sulfaguanidine, and lithium chloride. The sulfhydryl inhibitors, p-chloromercuribenzoate, and iodoacetate inhibited spherulation and this inhibition was reversed by the addition of glutathione. The anionic surface active compound, Tamol "N," was more effective in stimulating growth, spherulation, spherule rupture, and dispersal of endospores than any other surface active compound tested. A combination of Tamol "N" and sodium fluoride resulted in practically pure suspensions of intact spherules.