Inhibition of Haemopoietic Stem Cell Proliferation by a Diffusible Product of Bone Marrow Cells

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Abstract
In a double diffusion chamber (DC) culture system, [mouse] bone marrow cells elaborated diffusible factor(s) that prevented spleen colony-forming cells (CFU-S), but not PHA [phytohemagglutinin] stimulated lymphocytes, from entering cell cycle. Mature granulocytes and macrophages did not produce such factor(s). Various numbers of steady-state or regenerating mouse bone marrow cells were cultured in single diffusion chambers for periods up to 7 d. After the initial cell loss, the net growth of CFU-S was inversely related to total cell number and CFU-S number in DC. Diffusible factors rather than cell-to-cell contact appeared to be involved in the inhibition, even though it was not possible to demonstrate inhibition of net growth of CFU-S with the double chamber approach.