DILUTE UNMORDANTED HEMATOXYLIN AS A STAIN FOR BASIC NUCLEAR PROTEIN

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Abstract
Nucleic acids were removed from formalin-fixed, paraffin-embedded sections of numerous mouse organs by acid hydrolysis resulting in negative Feulgen and azure A stained nuclei. Nuclei progressively acquired stainability with unmordanted hematoxylin with increasing acid hydrolysis, and maximum staining occurred in nuclei devoid of nucleic acids. The recommended stain for nucleoprotein follows: sections are deparaffinized; transferred to absolute alcohol for 3 min.; collodionized; hydrolyzed for 1 hr. in 1 N HC1; rinsed; stained in 0.1% unmordanted hematoxylin in 0.2 N Iris buffer pH 8.2 for 1 hr. at 37[degree]C; dehydrated and mounted in Permount.

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