Origin and Direction of Replication of the Chromosome of E. coli B/r
- 1 March 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 65 (3) , 601-608
- https://doi.org/10.1073/pnas.65.3.601
Abstract
The origin and direction of replication of the E. coli B/r chromosome has been determined by comparing gene frequencies in P1-transducing lysates prepared on cultures growing at different rates. The gene frequencies found are consistent with the idea that replication of the chromosome is dichotomous in rapidly growing B/r. The origin was found to be between 40 and 55 min on the E. coli genetic map with replication proceeding in a clockwise direction. Markers near the origin behaved anomalously.Keywords
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