RNA-binding proteins that inhibit RNA virus infection
- 27 February 2007
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 104 (9) , 3129-3134
- https://doi.org/10.1073/pnas.0611617104
Abstract
Arrays of >5,000 Saccharomyces cerevisiae proteins were screened to identify proteins that can preferentially bind a small RNA hairpin that contains a clamped adenine motif (CAM). A CAM is required for the replication of Brome Mosaic Virus (BMV), a plant-infecting RNA virus that can replicate in S. cerevisiae. Several hits were selected for further characterization in Nicotiana benthamiana. Pseudouridine Synthase 4 (Pus4) and the Actin Patch Protein 1 (App1) modestly reduced BMV genomic plus-strand RNA accumulation, but dramatically inhibited BMV systemic spread in plants. Pus4 also prevented the encapsidation of a BMV RNA in plants and the reassembly of BMV virions in vitro. These results demonstrate the feasibility of using proteome arrays to identify specific RNA-binding proteins for antiviral activities. Furthermore, the effects of Pus4 suggest that the CAM-containing RNA motif provides a regulatory link between RNA replication and encapsidation.Keywords
This publication has 54 references indexed in Scilit:
- Selective Repression of Translation by the Brome Mosaic Virus 1a RNA Replication ProteinJournal of Virology, 2007
- Saccharomyces cerevisiae: A useful model host to study fundamental biology of viral replicationVirus Research, 2006
- Pathogen Recognition and Innate ImmunityCell, 2006
- BROMEMOSAICVIRUSRNA REPLICATION: Revealing the Role of the Host in RNA Virus ReplicationAnnual Review of Phytopathology, 2003
- The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromovirusesArchiv für die gesamte Virusforschung, 2003
- Brome Mosaic Virus Protein 1a Recruits Viral RNA2 to RNA Replication through a 5′ Proximal RNA2 SignalJournal of Virology, 2001
- Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoterJournal of Molecular Biology, 2001
- A minimal RNA promoter for minus-strand RNA synthesis by the brome mosaic virus polymerase complexJournal of Molecular Biology, 1999
- Acetyltransfer Precedes Uridylyltransfer in the Formation of UDP-N-acetylglucosamine in Separable Active Sites of the Bifunctional GlmU Protein of Escherichia coliBiochemistry, 1996
- RNA-dependent replication, transcription, and persistence of brome mosaic virus RNA replicons in S. cerevisiaeCell, 1993