INVITRO AND INVIVO DETECTION OF INFECTIOUS PANCREATIC NECROSIS VIRUS IN FISH BY ENZYME-LINKED IMMUNOSORBENT-ASSAY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 45  (9) , 1876-1879
Abstract
A double antibody ELISA was used to detect infectious pancreatic necrosis virus (IPNV). The ELISA detected VR299 strain of IPNV at a dose of 10 to 20 ng of purified IPNV protein or 104 TCID50 [median tissue culture infective dose] in tissue culture fluid. Specificity of ELISA was demonstrated by an ELISA inhibition test. The ELISA did not detect infectious hematopoietic necrosis virus. Normal cell culture fluid and virus-non-inoculated rainbow trout (Salmo gairdneri) hmogenate did not react in the test system. The IPNV was detected in rainbow trout fry inoculated with IPNV. Although infective virus titer in fish decreased rapidly 1 wk after inoculation, IPNV antigen was detected by ELISA for 15 days. The IPNV antigen was detected in the fish tissue after inactivation of infective virus. The ELISA is a rapid and reliable method for the diagnosis of IPNV infection. [Implications with respect to the shedding of the virus by infected fish and the resultig contamination of the field population are presented.].

This publication has 0 references indexed in Scilit: