FosB, a Cysteine-Dependent Fosfomycin Resistance Protein under the Control of ς W , an Extracytoplasmic-Function ς Factor in Bacillus subtilis

Abstract
We demonstrate that the Bacillus subtilis fosB(yndN)gene encodes a fosfomycin resistance protein. Expression offosB requires ςW, and both fosBand sigW mutants are fosfomycin sensitive. FosB is a metallothiol transferase related to the FosA class of Mn2+-dependent glutathione transferases but with a preference for Mg2+ and l-cysteine as cofactors.